Data sheet
| Assay Range | 62.5 - 4000 pg/ml |
More info
Sensitivity
50 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human DPP7 standard: 5 ng/vial - 2.
2. One 96-well plate precoated with anti-human DPP7 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human DPP7 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Dipeptidyl-peptidase II (DPPII), also known as quiescent cell proline dipeptidase (QPP) and dipeptidyl-peptidase 7(DPP7), is a post-proline cleaving amino peptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH. DPP7 plays an important role in the degradation of some oligopeptides.
