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Human DLL1 ELISA Kit

NS-E10060

$576.00

Data sheet

Assay Range 46.875 - 3000 pg/ml

More info

Sensitivity
40 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant human DLL1 standard: 3 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-human DLL1 Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Human DLL1 Ab: 1 vial, dilution 1:1000.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

DLL1 (Delta-like protein 1) is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It is expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. It acts as a ligand for Notch receptors and plays a role in mediating cell fate decisions during hematopoiesis.


 Standard Curve

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