Data sheet
| Assay Range | 4.68 - 300 pg/ml |
More info
Sensitivity
4 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD86 standard: 1 ng/vial - 2.
2. One 96-well plate precoated with anti-human CD86 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD86 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides cost imulatory signals necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 (for auto regulation and intercellular association) andCTLA-4 (for attenuation of regulation and cellular disassociation). CD86 works in tandem with CD80 to prime T cells.
