Human CD32b ELISA Kit ( Fc-gamma-RIIb/ FCGR2B )
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  • Human CD32b ELISA Kit ( Fc-gamma-RIIb/ FCGR2B )

Human CD32b ELISA Kit ( Fc-gamma-RIIb/ FCGR2B )

Catalog # NS-E10031

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$625.00
Quantity

For research use only. Not for diagnostic or therapeutic use, or for human or veterinary use. Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.
Sensitivity
15 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant human CD32b standard: 2 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-human CD32b Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Human CD32b  Ab: 1 vial, dilution 1:500.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

CD32b, also known as FCGR2B, is a "low affinity" receptor for Immunoglobulin G. The inhibitory CD32b is expressed on B cells and myeloid dendritic cells.   CD32b is the inhibitory FcR and the inhibitory capacity of CD32b is largely dependent on expression of an intracellular ITIM. Ligation of CD32b on B cells downregulates antibody production and may promote apoptosis. Co-ligation of CD32b on dendritic cells inhibits maturation and blocks cell activation. CD32b may also be a target for monoclonal antibody therapy for malignancies. Deficiency in CD32b leads to chronic B cell activation and autoimmunity. Polymorphisms in CD32b are associated with an increased prevalence of SLE.

 

Standard Curve

Data sheet

Assay Range
31.2 - 2000 pg/ml
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