Human Cadherin-1 ELISA Kit ( E-cadherin/ CDH1/ CD324 ) (NS-E10017)
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  • Human Cadherin-1 ELISA Kit ( E-cadherin/ CDH1/ CD324 ) (NS-E10017)

Human Cadherin-1 ELISA Kit ( E-cadherin/ CDH1/ CD324 )

Catalog # NS-E10017

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$625.00
Quantity

Sensitivity
20pg/mL 
 
Size 
96T
 
Assay Principle
Sandwich ELISA 
 
Storage 
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Sample Volume
100 µL final volume, dilution factor varies on samples
 
Detection Method
Chromogenic 
 
 
Kit Components
 
 
 
1. Recombinant Human Cadherin-1 standard: 10ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-Human Cadherin-1 Ab.
 
 
3. Sample diluent buffer: 12 ml
 
 
4. Biotin conjugated anti-Human Cahderin-1 Ab: 1 vial, dilution 1:1500.
 
 
5. Detection Antibody diluent buffer: 12ml.
 
                                                  6. Strepavidin-HRP: 1 vial, dilution 1:200.
 
 
7. Strepavidin-HRP dilution buffer : 12 ml.
 
 
8. TMB color developing agent A: 6 ml.
 
 
9. TMB color developing agent B: 6 ml.
 
 
10. TMB stop solution: 6ml.
 
 
 
  
Background

Cadherin-1, also known as CDH1or epithelial cadherin (E-cadherin) is a classical member of the cadherin superfamily. Cadherins are calcium-dependent cell adhesion proteins. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells with a potent invasive suppressor role. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis.


Standard Curve

 

Data sheet

Assay Range
46.88 - 3000 pg/ml
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