Data sheet
| Assay Range | 78.125 - 5000 pg/ml |
More info
Sensitivity
50pg/mL
Size
96T
Assay Principle
Sandwich ELISA
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant Human C1s standard: 10ng/vial - 2.
2. One 96-well plate precoated with anti-Human C1s antibody.
3. Sample diluent buffer: 12 ml
4. HRP conjugated anti-Human C1s antibody: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12ml.
6. Assay diluent buffer: 12 ml.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
C1s is a serine protease, which is a major constituent of the human complement subcomponent C1. C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. C1s associates with C1r and C1q in order to yield the first component of the serum complement system so that it can, in turn, activate C2 and C4.Defects in C1s are the cause of complement component C1s deficiency (C1SD).
