View larger
Deoxyribonuclease I Bovine ( DNase Bovine )
SourceExtracted from Pancreas.Physical AppearanceSterile lyophilized freezed dried powder.Stability2 years when stored at 4°C, three weeks at 15°C, pH-6.3.Specific Activity316IU/1mg.Unit DefenitionOne unit will produce a A260 of 0.001/min/mL
Data sheet
| Protein Background | Deoxyribonuclease I Bovine (bDNase), an enzyme which selectively cleaves DNA. Bovine Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarely on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2 .Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments. |
| Expression host | Extracted from Pancreas. |
| Synonyms | EC 3.1.21.1, Deoxyribonuclease I, DNase I, DNL1, DRNI, FLJ38093, DNASE1, Deoxyribonuclease-1. |
| Reagent Appearance | Sterile lyophilized freezed dried powder. |
| Unit Definition | One unit will produce a A260 of 0.001/min/mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C. |
| Specific Activity | 316IU/1mg. |