Fibroblast Growth Factor-21 Bovine Recombinant ( FGF 21 Bovine )

Data sheet

Formulation

The protein was lyophilized from a concentrated (0.8 mg/ml) solution with 0.4 mg/ml of NaHCO3, pH 8.

Solubility

It is recommended to reconstitute the lyophilized Bovine FGF-21 in sterile water or 0.4% NaHCO3, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Purity

Greater than 98.0% as determined by:(a) Analysis by Gel Filtration.(b) Analysis by SDS-PAGE.

Description

Fibroblast Growth Factor -21 Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 182 amino acids, having a molecular weight of 19.5 kDa.The FGF-21 is purified by proprietary chromatographic techniques.

Protein Background

The FGFs are a family of more than 20 small (~1726 kDa) secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors (FGFRs) 1,

Expression host

Escherichia Coli.

Synonyms

Fibroblast growth factor 21, FGF-21, FGF21.

Reagent Appearance

Sterile Filtered white lyophilized powder.

Stability

Lyophilized FGF-21 Bovine Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor 21 should be stored at 4°C between 2-7 days and for future use below -18°C.For lon

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-His-Pro-Ile-Pro.