Granulocyte-Colony Stimulating Factor Human Recombinant ( G CSF Human ) (PT_40629)
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  • Granulocyte-Colony Stimulating Factor Human Recombinant ( G CSF Human ) (PT_40629)

Granulocyte-Colony Stimulating Factor Human Recombinant ( G CSF Human )

Catalog # PT_40629

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$201.00
DescriptionGranulocyte Colony Stimulating Factor Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 KD.GCSF is purified by proprietary chromatographic
Product Size: 2ug
Quantity

Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.

Granulocyte Colony Stimulating Factor Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 KD.GCSF is purified by proprietary chromatographic techniques.

  • Expression host: Escherichia Coli.
  • Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
  • Biological activity: The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is
  • Formulation: GCSF was lyophilized after extensive dialysis against 10mM sodium acetate buffer pH= 4.
  • Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
  • Stability: Lyophilized GCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a ...

Catalog number PT_40629. Available sizes: 2 ug, 10 ug, 1 mg. Order online or request a quote. For research use only. Not for diagnostic or therapeutic use, and not for human or animal consumption.

Data sheet

Formulation
GCSF was lyophilized after extensive dialysis against 10mM sodium acetate buffer pH= 4.
Solubility
It is recommended to reconstitute the lyophilized GCSF in sterile 20mM AcOH not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Purity
Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Description
Granulocyte Colony Stimulating Factor Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 KD.GCSF is purified by proprietary chromatographic techniques.
Protein Background
GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for the GCSF gene. Granulocyte/macroph
Expression host
Escherichia Coli.
Synonyms
CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.
Reagent Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized GCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a ca
Amino acid sequence
The sequence of the first five N-terminal amino acids of GCSF was determined and was found to be Met-Thr-Pro-Leu-Gly.
Biological Activity
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is
References
Title:Contribution of an Aged Microenvironment to Aging-Associated Myeloproliferative Disease.Publication:Vas V, Wandhoff C, Drr K, Niebel A, Geiger H (2012) Contribution of an Aged Microenvironment to Aging-Associated Myeloproliferative Disease. PLoS ONE
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