Hydroxyacid Oxidase 1 Human Recombinant ( HAO1 Human ) (PT_73874)
search
  • Hydroxyacid Oxidase 1 Human Recombinant ( HAO1 Human ) (PT_73874)

Hydroxyacid Oxidase 1 Human Recombinant ( HAO1 Human )

Catalog # PT_73874

Print

$201.00
DescriptionHAO1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 406 amino acids (1-370 a.a.) and having a molecular mass of 45kDa.HAO1 is fused to a 36 amino acid His-tag at N-terminus & purified by
Product Size: 5ug
Quantity

Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.

HAO1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 406 amino acids (1-370 a.a.) and having a molecular mass of 45kDa.

  • Expression host: Escherichia Coli.
  • Purity: Greater than 95% as determined by SDS-PAGE.
  • Formulation: HAO1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 0.5M NaCl.
  • Appearance: Sterile filtered colorless solution.
  • Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Catalog number PT_73874. Available sizes: 5 ug, 20 ug, 1 mg. Order online or request a quote. For research use only. Not for diagnostic or therapeutic use, and not for human or animal consumption.

Data sheet

Formulation
HAO1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 0.5M NaCl.
Purity
Greater than 95% as determined by SDS-PAGE.
Description
HAO1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 406 amino acids (1-370 a.a.) and having a molecular mass of 45kDa.HAO1 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromat
Protein Background
Glycolate oxidase (HAO1) belongs to the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. HAO1 catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. HAO1 is mos
Expression host
Escherichia Coli.
Synonyms
Hydroxyacid oxidase 1, HAOX1, Glycolate oxidase, GOX, HAO1, GOX1.
Reagent Appearance
Sterile filtered colorless solution.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Amino acid sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRML RNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLV RQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PE
Storage buffer
Km=15.1uM, Kcat = 30s-1, Kcat/Km= 1981 mM-1s-1 with peptide substrate KARVF (NO2)VRKA (F(NO2) ... p-nitrophenylalanine).
New