Data sheet
| Assay Range | 0.78 – 50 pg/mL |
| Size | 96T |
| Storage | 2 - 8 ℃ |
| Background | IL-22 is an α-helical cytokine. IL-22 binds to a heterodimeric cell surface receptor composed of IL-10R2 and IL-22R1 subunits.IL-22R is expressed on tissue cells, and it is absent on immune cells. IL-22 is produced by several populations of immune cells at a site of inflammation. Producers are αβ T cells classes Th1, Th22 and Th17 along with γδ T cells, NKT, ILC3, neutrophils and macrophages. IL-22 takes effect on non-hematopoietic cells – mainly stromal and epithelial cells. Effects involve stimulation of cell survival, proliferation and synthesis of antimicrobials including S100, Reg3β, Reg3γ and defensins. IL-22 thus participates in both wound healing and in protection against microbes. IL-22 dysregulation takes part in pathogenesis of several autoimmune diseases like systemic lupus erythematosus, rheumatoid arthritis and psoriasis. |
| Sample | 5-10 µL mouse serum/plasma |
| Alternate Names | IL-21; IL22; IL-22; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18 |
| Assay Type | Sandwich ELISA |
| Kit Type | Colorimetric |
| Gene ID | 116849 |
| UniProt ID | Q9JJY8 |
More info
Storage
Store at 4 °C.
Expiration
6 months at 4 °C.
Range
0.78 pg/mL - 50 pg/mL.
Sensitivity
0.31 pg/mL.
Specificity
No detectable cross-reactivity with any other cytokine.
Application
cell culture supernatants, serum and plasma.
Kit Components
1. Lyophilized recombinant Mouse IL-22 standard: 50 pg/tube-2.
2. One 96-well plate precoated with anti-Mouse IL-22 antibody.
3. Sample diluent buffer: 12 mL —2
4. Biotinylated anti-Mouse IL-22 antibody: 60µL, dilution 1:180.
5. Antibody diluent buffer: 12ml.
6. Streptavidin-HRP: 300 µL dilution 1:40.
7. Streptavidin-HRP diluent buffer: 12ml.
8. Chromogen-A, 6ml; Chromogen-B, 6ml.
9. Stop Solution: 6ml.