Data sheet
| Assay Range | 0.78 – 50 pg/mL |
| Size | 96T |
| Background | Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. This gene encodes a subunit of the cytokine Interleukin 12 (IL-12) that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-dependent induction of interferon gamma (INF-γ), and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. |
| Sample | 5-10 µL mouse serum/plasma |
| Alternate Names | IL12B, CLMF, CLMF2, IL-12B, IMD28, NKSF, NKSF2, IMD29, Interleukin 12 subunit beta, interleukin 12B |
| Gene ID | 16160 |
| UniProt ID | P43432 |
More info
Range
0.78 pg/mL-50 pg/mL
Sensitivity
0.25 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 °C for frequent use, at -20 °C for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
6 months at 4 °C and 12 months at -20 °C.
Application
serum, body fluids, tissue lysate, cell culture supernates.
Kit Components
1. Lyophilized recombinant mouse IL-12(p70) standard: 50
pg/tube-2.
2. One 96-well plate precoated with anti- mouse IL-12(p70)
antibody.
3. Sample diluent buffer: 12 ml x 2
4. Biotinylated anti- mouse IL-1a antibody: 60 µL, dilution 1:180.
5. Antibody diluent buffer: 12ml.
6. Strepavidin-HRP: 300µL, dilution 1:40.
7. Strepavidin-HRPdiluent buffer: 12ml.
8. Chromogen-A, 6 ml; Chromogen-B, 6 ml.
9. Stop Solution: 6 ml.