Data sheet
| Assay Range | 0.78 – 50 pg/mL |
| Sensitivity | 0.5 pg/mL |
| Size | 96T |
| Storage | 2 - 8 ℃ |
| Application | Save mouse plasma and serum samples. Generate high-sensitivity detection assay |
| Background | Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6's role as an anti-inflammatory myokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. IL-6 is also considered a myokine, a cytokine produced from muscle, which is elevated in response to muscle contraction. It is significantly elevated with exercise, and precedes the appearance of other cytokines in the circulation. During exercise, it is thought to act in a hormone-like manner to mobilize extracellular substrates and/or augment substrate delivery. IL-6 had previously been classified as a proinflammatory cytokine. Therefore, it was first thought that the exercise-induced IL-6 response was related to muscle damage.However, it has become evident that eccentric exercise is not associated with a larger increase in plasma IL-6 than exercise involving concentric "nondamaging" muscle contractions. This finding clearly demonstrates that muscle damage is not required to provoke an increase in plasma IL-6 during exercise. As a matter of fact, eccentric exercise may result in a delayed peak and a much slower decrease of plasma IL-6 during recovery. |
| Sample | 5-10 µL mouse serum/plasma |
| Alternate Names | IL6, BSF2, HGF, HSF, IFNB2, IL-6, BSF-2, CDF, IFN-beta-2, interleukin 6 |
| Gene ID | 16193 |
| UniProt ID | P08505 |
More info
Kit Components:
1. Lyophilized recombinant Mouse IL-6 standard: 50 pg/tube—2.
2. One 96-well plate precoated with anti-Mouse IL-6 antibody.
3. Sample diluent buffer: 12 mL —2
4. Biotinylated anti-Mouse IL-6 antibody: 60µL, dilution 1:180.
5. Antibody diluent buffer: 12ml.
6. Streptavidin-HRP: 300 µL, dilution 1:40.
7. Streptavidin-HRP diluent buffer: 12ml.
8. Chromogen A: 6ml.
9. Chromogen B: 6ml.
10. Stop Solution: 6ml.