| Formulation |
SPRR1B filtered (0.4 um) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4. |
| Solubility |
It is recommended to add 200ul deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. SPRR1B is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Description |
SPRR1B Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 2-89) containing 97 amino acids including a 9 a.a N-terminal His tag. The total molecular mass is 10.9kDa (calculated). |
| Protein Background |
Small Proline-Rich Protein 1B (SPRR1B) is a keratinocyte protein which first appears in the cell cytosol, but eventually becomes cross-linked to membrane proteins by transglutaminase. SPRR1B is cross-linked to membrane proteins by transglutaminase leading to formation of an insoluble envelope. SPRR1B functions as both amine donor and acceptor in transglutaminase-mediated cross-linkage. SPRR1B is expressed in the course of differentiation in squamous tissues such as epidermis, esophagus, tongue or trachea. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Cornifin-B, 14.9 kDa pancornulin, Small proline-rich protein IB, SPR-IB, SPRR1B, SPRR1, GADD33, CORNIFIN. |
| Reagent Appearance |
Filtered White lyophilized (freeze-dried) powder. |
| Stability |
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Amino acid sequence |
MKHHHHHHAS SQQQKQPCTP PPQLQQQQVK QPCQPPPQEP CIPKTKEPCH PKVPEPCHPK VPEPCQPKVP EPCHPKVPEP CPSIVTPAPA QQKTKQK. |
