| Formulation |
CTLA4 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Description |
CTLA4 Mouse Recombinant produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 363 amino acids (38-161aa) and having a molecular mass of 40.6kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).CTLA4 is fused to a 239 amino acid hIgG-His-tag at C-terminus & purified by proprietary chromatographic techniques. |
| Protein Background |
CTLA-4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. |
| Expression host |
Sf9 Insect cells. |
| Synonyms |
Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, Ctla4, Cd152. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
IQVTQPSVVL ASSHGVASFP CEYSPSHNTD EVRVTVLRQT NDQMTEVCAT TFTEKNTVGF LDYPFCSGTF NESRVNLTIQ GLRAVDTGLY LCKVELMYPP PYFVGMGNGT QIYVIDPEPC PDSDLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHEDPEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHHHHH. |
| Rapid Test Protocol |
Purified IgG. |
