| Reconstitution |
1mg/ml in PBS (after reconstitution). |
| Formulation |
CD34 protein solution (0.25mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol. |
| Purity |
Greater than 95.0% as determined by SDS-PAGE. |
| Description |
CD34 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 268 amino acids (32-290a.a.) and having a molecular mass of 28.6kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).CD34 is fused with a 6 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques. |
| Protein Background |
CD34 is a single chain transmembrane glycoprotein which is selectively expressed on human lymphoid and myeloid hemapoietic progenitor cells. CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. |
| Expression host |
Sf9, Baculovirus cells. |
| Synonyms |
CD34 molecule, Hematopoietic progenitor cell antigen CD34, CD34 Antigen, CD34. |
| Reagent Appearance |
Sterile filtered colorless solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
ADLSLDNNGT ATPELPTQGT FSNVSTNVSY QETTTPSTLG STSLHPVSQH GNEATTNITE TTVKFTSTSV ITSVYGNTNS SVQSQTSVIS TVFTTPANVS TPETTLKPSL SPGNVSDLST TSTSLATSPT KPYTSSSPIL SDIKAEIKCS GIREVKLTQG ICLEQNKTSS CAEFKKDRGE GLARVLCGEE QADADAGAQV CSLLLAQSEV RPQCLLLVLA NRTEISSKLQ LMKKHQSDLK KLGILDFTEQ DVASHQSYSQ KTHHHHHH. |
| Antibody Properties |
Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Available Conjugates |
Cytotoxic and staining antibody. For staining, use 10ul/1,000,000 cells. Exact titer for cytotoxicity should be determined by the investigator. |
| Immunological function |
This antibody is only available as purified antibody. |
