| Formulation |
The ANAPC13 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 1mM DTT and 0.1M NaCl. |
| Purity |
Greater than 90.0% as determined by SDS-PAGE. |
| Description |
ANAPC13 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 89 amino acids (1-74) and having a molecular mass of 10kDa (Molecular weight on SDS-PAGE will appear higher).ANAPC13 protein is fused to a 15 amino acid T7-tag at N-terminus and is purified by standard chromatography. |
| Protein Background |
Anaphase-promoting complex subunit 13 (ANAPC13) is a component of the anaphase promoting complex, which is a large ubiquitin-protein ligase that controls cell cycle progression by regulating the degradation of cell cycle regulators such as B-type cyclins. The ANAPC13 protein is evolutionarily conserved and is essential for the integrity and ubiquitin ligase activity of the anaphase promoting complex. |
| Expression host |
Escherichia Coli. |
| Synonyms |
Anaphase-promoting complex subunit 13, APC13, Cylosome subunit 13, ANAPC13, SWM1. |
| Reagent Appearance |
Sterile Filtered clear solution. |
| Stability |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence |
MASMTGGQQM GRGSHMDSEV QRDGRILDLI DDAWREDKLP YEDVAIPLNE LPEPEQDNGG TTESVKEQEM KWTDLALQYL HENVPPIGN. |
