Data sheet
| Assay Range | 23.4 - 1500 pg/ml |
More info
Sensitivity
20 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Human IL-32 standard: 3 ng/vial - 2.
2. One 96-well plate precoated with anti-Human IL-32 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human IL-32 Ab: 1 vial, dilution 1:400.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Interleukin 32 (IL-32) is a pro-inflammatory cytokine that can induce cells of the immune system to secrete inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and IL-6. IL-32 is selectively expressed in lymphocytes and the expression is increased after activation of T-cells by mitogens or activation of NK cells by IL2. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN-gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn induces cytokine expression. In addition, it can also induce the production of chemokines such as IL-8 and MIP-2 / CXCL2. IL-32 may play a role in both innate and adaptive immune responses. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
