Mouse EpCAM / TROP-1 / TACSTD1 ELISA Kit
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  • Mouse EpCAM / TROP-1 / TACSTD1 ELISA Kit

Mouse EpCAM / TROP-1 / TACSTD1 ELISA Kit

Catalog # NS-E10245

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$625.00
Quantity

For research use only. Not for diagnostic or therapeutic use, or for human or veterinary use. Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.
Sensitivity
50 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant Mouse EpCAM standard: 10 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-Mouse EpCAM Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Mouse EpCAM Ab: 1 vial, dilution 1:1000.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+ independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. EpCAM is expressed on the basolateral membrane of all simple (especially glandular), pseudo-stratified, and transitional epithelia.Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies. 

Standard Curve

Data sheet

Assay Range
78 - 5000 pg/ml
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