Mouse CD48 / SLAMF2 / BCM1 ELISA Kit
Catalog # NS-E10241
$625.00
For research use only. Not for diagnostic or therapeutic use, or for human or veterinary use. Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.
Sensitivity
50 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse CD48 standard: 5 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse CD48 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse CD48 Ab: 1 vial, dilution 1:800.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF) which includes SLAM (signaling lymphocyte activation molecules) proteins. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. CD48 was the first B-cell-specific cellular differentiation antigen identified in transformed B lymphoblasts. CD48 and CD2 molecular coupling together with other interaction pairs of CD28 and CD80, TCR and peptide-MHC and LFA-1 and ICAM-1 contribute to the formation of an immunological synapse between a T cell and an antigen presenting cell. CD48 interaction with CD2 has been shown to promote lipid raft formation, T cell activation and the formation of caveolae for macrophages through cell signal transductionthe via GPI moieties.
Data sheet
- Assay Range
- 62.5 - 4000 pg/ml
New