More info
Assay range | 2.5-80 ng/ml |
Sensitivity | 2.5 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | plasma, serum, milk, tissue extract, and cell culture lysates. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human HSP60 standard: 1 vial
2. One 96-well plate coated withHuman HSP60 Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human HSP60 Ab (70x): 105µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Heat shock protein 60 (HSP60), also knwon as 60 kDa chaperonin, Chaperonin 60, CPN60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, or HSPD1, is a 573 amino acid molecular chaperone and is highly homologous to the bacteria groEL protein. HSP60 exists in the mitochondria and cytoplasm, the cell surface, the extracellular space, and the peripheral blood. HSP60 is a mitochondrial chaperonin involved in folding, assembly, and transport of newly imported protein from cytoplasm into mitochondria, in addition to protecting cells from heat shock. HSP60 has been implicated in myelinogenesis, neurodegenerative diseases, diabetes, cancer, and immunological disorders.