Human CD82 / KAI-1 ELISA Kit (NS-E10217)
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  • Human CD82 / KAI-1 ELISA Kit (NS-E10217)

Human CD82 / KAI-1 ELISA Kit

Catalog # NS-E10217

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$625.00
Quantity

For research use only. Not for diagnostic or therapeutic use, or for human or veterinary use. Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.
Sensitivity
70 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant human CD82 standard:5 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-human CD82 Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Human CD82 Ab: 1 vial, dilution 1:700.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

The transmembrane glycoprotein CD82, also known as Kangai (KAI)-1,is a membrane glycoprotein that is a member of  transmembrane 4 superfamily.  CD82 is lymphoid specific and associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. Expression of CD82 has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. The reduced KAI-1 expression may be the result of dysregulated p53 function and could be an important step in the endometrial carcinogenesis. 

standard curve

Data sheet

Assay Range
78 - 5000 pg/ml
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