Human XPNPEP2 ELISA Kit (NS-E10209)
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  • Human XPNPEP2 ELISA Kit (NS-E10209)

Human XPNPEP2 ELISA Kit

Catalog # NS-E10209

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$625.00
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For research use only. Not for diagnostic or therapeutic use, or for human or veterinary use. Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.
Sensitivity
10 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant human XPNPEP2 standard:1 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-human XPNPEP2 Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Human XPNPEP2 Ab: 1 vial, dilution 1:1000.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

The membrane-bound X-prolyl aminopeptidase (aminopeptidase P) 2, also known as XPNPEP2, belongs to the peptidase M24B family, and has been reported to associate with Aminopeptidase P (APP) activity.  XPNPEP2 is expressed in kidney, lung, heart, placenta, liver, small intestine and colon. No expression in brain, skeletal muscle, pancreas, spleen, thymus, prostate, testis and ovary. It can release any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide. XPNPEP2 is a metalloprotease that may play a role in the inflammatory process and other reactions produced in response to injury or infection. XPNPEP2 may also play a role in the metabolism of the vasodilator bradykinin.

Standard Curve

Data sheet

Assay Range
11 - 700 pg/ml
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