Data sheet
| Assay Range | 47 - 3000 pg/ml |
More info
Sensitivity
40 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human ACBD6 standard:10 ng/vial - 2.
2. One 96-well plate precoated with anti-human ACBD6 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human ACBD6 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Human Acyl-CoA-binding domain-containing protein 6, also known as ACBD6 is a Cytoplasm modular protein that carries an acylCoA binding domain at its N terminus and two ankyrin motifs at its C terminus. It can be detected in placenta, spleen placenta, umbilical cord blood, CD34-positive hematopoietic progenitor cells and bone marrow. ACBD6 binds long-chain acyl-coenzyme A molecules with a strong preference for unsaturated C18:1-CoA, lower affinity for unsaturated C20:4-CoA, and very weak affinity for saturated C16:0-CoA. It does not bind fatty acids. Human ACBD6 may represent a cellular marker for primitive progenitor cells with functions in hematopoiesis and vascular endothelium development.
