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MHC Class II, ( I-A ) Mouse Antibody ( MHC Class II, I-A Antibody )
TypeMouse Antibody Monoclonal.CloneNYRmI-A.ImmunogenPurified mouse LN B cells (C3H anti-C57Bl6).Ig SubclassMouse IgG2a.SpecificityRecognizes most MHC class II haplotypes (b,f,p,q,r,s,u,v), but weak against I-Ak.Purification MethodIon exchange
Data sheet
| Clone | NYRmI-A. |
| Protein Background | MHC Class II is consists of 2 membrane spanning proteins. Each of these proteins is roughly 30 kDa, and has of two Globular domains, Alpha-1, Alpha-2, Beta-1 and Beta-2. The two regions farthest away from the membrane are alpha-1 and beta-1. The two proteins link without Covalent Bonds. The MHC Class II protein mainly presents peptides, which have been digested from external sources. MHC Class II is normally only expressed by Antigen Presenting Cells (APC) which digest foreign protein. Within the RER the alpha and beta proteins of the molecule, associate with each other, while a third protein called the "invariant chain," is necessary for stabilizing the complex, without it, they will not associate. The MHC-invariant complex is then passed from the RER, into and out of, the Golgi body. It fuses with an Endocytic compartment, where an external protein has been sampled and degraded. |
| Type | Mouse Antibody Monoclonal. |
| Immunogen | Purified mouse LN B cells (C3H anti-C57Bl6). |
| Ig Subclass | Mouse IgG2a. |
| Purification Method | Ion exchange column. |
| Storage Procedures | Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. |