Data sheet
| Background | Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a member of the common gamma chain cytokine family. The 153 amino acid (aa) human IL-2 precursor contains a 20 aa signal sequence and a 133 aa mature region with one O-linked glycosylation site at Thr3, plus three cysteines, two of which form an intrachain disulfide bond that is essential for IL-2 bioactivity. Human IL-2 shares approximately 60% sequence identity with mouse IL-2, and is functional on mouse IL-2 responsive cells. IL-2 is expressed and secreted on γδT cells, activated conventional CD4+ and CD8+ T cells, neurons, microglia, and hematopoietic stem cells in humans. IL-2 first binds to IL-2 receptor alpha (CD25) in the binary receptor complex then recruits IL-2Rbeta/CD122 and IL-2Rbeta/CD132 to trigger a variety of biological processes. IL-2 plays a key role in the development, maintenance and function of regulatory T cells and in promoting the development of activated CD8+ T cells into memory cells. |
| Alternate Names | aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF |
| Assay Type | Sandwich ELISA |
| Kit Type | Colorimetric ELISA |
| Assay Time | 4.5 |
| Sample Type | Plasma, Serum, Cell culture medium |
| Assay Range | 46.9-3000 pg/ml |
| Sensitivity | 5 pg/ml |
| Specificity | This kit recognizes porcine Il-2 in samples. No significant cross-reactivity or interference between porcine IL-2 and analogues was observed. |
| Recovery | 95-108% |
| Precision | Intra Assay, 5.3%; Inter-Assay, 8.8%; |
| Sample Volume | 100 ul |
| Dilution Factors | Varies. Need to test before a large-scale assay. |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Gene ID | 396868 |
| UniProt ID | P26891 |
More info
Kit Components
1. Recombinant Porcine IL-2 standard: 2 vials
2. One 96-well plate coated with Porcine IL-2 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer:12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogenic solution A: 6 mlx1
9. Chromogenic solution B: 6 mlx1
10. Stop solution: 6 mL x1
11. Washing solution (20x): 25 mL x1