Uracil DNA Glycosilase ( UNG )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

UNG solution in 10mM Tris-HCl (pH-7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.

Description

E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).

Expression host

Escherichia Coli strain that carries the UNG gene from E.coli.

Synonyms

Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles.

Unit Definition

1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37°C.

Specific Activity

The Specific Activity was found to be 5U/ul.

Antibody Properties

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).