Data sheet
| Assay Range | 7.8 - 500 pg/ml |
More info
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse PIGR standard: 1 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse PIGR Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse PIGR Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
PIGR (Polymeric Immunoglobulin Receptor, pIgR) is a 100-kDa type I transmembrane glycoprotein. pIgR is expressed on secretory epithelial cells of exocrine tissues. It functions as a carrier that transports IgA and IgM across epithelium. This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface.
