| Specificity |
reactive with Mouse MBL-2，reactivity with other related proteins not observed |
| Assay Range |
31.25-2000 pg/ml |
| Sensitivity |
3 pg/ml |
| Size |
96 Tests |
| Storage |
2-8℃ |
| Background |
This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. |
| Assay principle |
This Mouse MBL-2 ELISA Kit was based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse MBL-2 specific antibodies were precoated onto 96-well plates. The Mouse MBL-2 specific detection antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow color is proportional to the Mouse MBL-2 amount of sample captured in plate. |
| Sample type |
serum，plasma，cell culture supernatant |
| Assay duration |
4 hours |
| Assay format |
Sandwich |
| Alternative Name of Analyte |
MBL；MBP；MBP1；MBPD；MBL2D；MBP-C；COLEC1；HSMBPC |
