More info
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Assay Range |
7.8 - 500 pg/mL |
|
Sensitivity |
1.0 pg/mL |
|
Size |
96T |
|
Storage |
Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
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Assay Principle |
Sandwich ELISA |
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Sample Volume |
100 µL final volume, dilution factor varies on samples |
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Detection Method |
Chromogenic |
Kit Components
1. Recombinant Mouse Mouse IL-3 ELISA Kit: 2 vials
2. One 96-well plate coated with Mouse IL-3 ELISA Kit Ab
3. Sample diluent buffer: 12 ml— 2
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Interleukin 3 (IL-3), also known as Hematopoietic growth factor, mast cell growth factor (MCGF), multi potential colony-stimulating factor, P-cell-stimulating factor, is a cytokine belonging to the IL-3 family. Human IL-3 precursor consists of a 19 aa signal peptide and a 133 (aa) mature polypeptide. Mouse IL-3 is synthesized as a 166 amino acid (aa) precursor protein containing a 26 aa hydrophobic signal peptide and a 140 aa mature protein. IL-3 is expressed by several hematopoietic and non-hematopoietic cell types including activated human T cells, natural killer cells, mast cells, epithelial cells, stromal cells, murine keratinocytes, neurons and astrocytes. Mouse IL-3 shares approximately 59% and 29% aa sequence identity with rat IL-3 and human IL-3, respectively, and shows little or no species cross-reactivity in rat and human. IL-3 binds to a heteromeric transmembrane receptor complex consisting of α (IL-3 Rα) and common β (βc) subunits. The βc subunit is shared with IL-5 and GM-CSF, while the IL-3 Rα subunit is specific for IL-3. Human IL-3 plays a key role in stimulating the proliferation, differentiation, and survival of pluripotent hematopoietic stem cells as well as various lineage-committed progenitors.