Data sheet
| Assay Range | 15.6 - 1000 pg/ml |
More info
Sensitivity
15 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse IL-18R1 standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse IL-18R1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse IL-18R1 Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
The interleukin-18 receptor 1 (IL18R1), also known as CDw218a (cluster of differentiation w218a), is a cytokine receptor that belongs to the interleukin 1 receptor family. It specifically binds interleukin 18 (IL18) and is essential for IL18 mediated signal transduction, and it is reported that binding to the agonist leads to the activation of NF-kappa-B. It modulates the function of many immunocompetent cells including macrophages, monocytes, lymphocytes and granulocytes. High level of IL18R1 expression is detected in Hodgkin's disease cell lines.
