Data sheet
| Assay Range | 24 - 1500 pg/ml |
More info
Sensitivity
20 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse FVII standard: 5 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse FVII Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse FVII Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Factor VII (formerly known as proconvertin) is one of the proteins that cause blood to clot in the coagulation cascade. The gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Defects in this protein can cause coagulopathy.
