Data sheet
| Assay Range | 6.25 - 400 pg/ml |
More info
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse CD5L standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse CD5L Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse CD5L Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD5L, also known as Sp alpha, is a secreted protein that is a member of the SCSR (scavenger receptor cysteine rich) superfamily. It is expressed in thymus, liver, spleen and lymph nodes which are associated with immunoglobulins of IgM isotype in the plasma/serum. CD5L/Sp alpha exhibits a high degree of homology with the extracellular regions of CD5 and CD6, exhibiting three SCSR domains and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. It may also play a role in the regulation of the immune system and work as an inhibitor of apoptosis.
