More info
Assay Range | 15.6-1,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse CCL24 standard: 2 vials
2. One 96-well plate coated withMouse CCL24 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Eotaxin-2, also known as CCL24, MPIF-2, or Ckβ-6, is a small cytokine belonging to the CC chemokine family. Eotaxin-2 mRNA is expressed in activated T lymphocytes, GM-CSF treated macrophages, and dermal fibroblasts. Human mature Eotaxin-2 is a 93 amino acid (aa) glycoprotein exhibiting relatively low aa identity with eotaxin. Mouse Eotaxin-2 is synthesized as a 119 (aa) precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Eotaxin-2 selectively recruits and activates eosinophils and basophils both in vitro and in vivo. Eotaxin-2 primarily binds to the G-protein-coupled receptor CCR3 expressed on eosinophils, basophils, subpopulations of Th2 lymphocytes, and keratinocytes to initiate a series of biological processes including actin polymerization, a transient rise in cytosolic calcium concentration, and release of reactive oxygen species. However, CCR3 is also a ligand for other chemokines such as Eotaxin, MCP-2, MCP-3, MCP-4, MIP-1δ, and RANTES. Eotaxin-2 has been implicated in certain pathological conditions linked to allergic reactions and parasitic infections.