Mouse SDF-1 Growth Factor ELISA Kit

Mouse SDF-1 Growth Factor ELISA Kit

FM-E100121

96T

62.5pg/mL-4000pg/mL

Less Than 10 pg/mL

No detectable cross-reactivity with any other cytokine.

Store at 4 for frequent use, at -20 for infrequent use.

Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Four months at 4 and eight months at -20.

For quantitative detection of mouse SDF-1 in serum,

plasma, body fluids, tissue lysates or cell culture supernates.

 

1. Lyophilized recombinant mouse SDF-1 standard: 10ng/tube-2.

2. One 96-well plate precoated with anti- mouse SDF-1 antibody.

3. Sample diluent buffer: 30 ml

4. Biotinylated anti- mouse SDF-1 antibody: 130µL, dilution 1:100.

5. Antibody diluent buffer: 12ml.

6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.

7. ABC diluent buffer: 12ml.

8. TMB color developing agent: 10ml.

9. TMB stop solution: 10ml.

 

 

SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). This gene is located on chromosome 10q11.1. SDF-1 is produced in two forms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes. CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes. CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide.8 The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.

 

Mouse SDF-1 ELISA Kit-1X96 Well Plate Image