Product name
Mouse IL-1beta ELISA Kit
Catalog No.
FM-E100055
Size
96T
Range
31.2pg/mL-2000pg/mL
Sensitivity
25 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
serum, plasma, body fluids or cell culture supernates.
Kit Components
1. Lyophilized recombinant mouse IL-1βstandard: 2ng/tube-2.
2. One 96-well plate precoated with anti- mouse IL-1β antibody.
3. Sample diluent buffer: 25 ml
4. Biotinylated anti- mouse IL-1β antibody : 60µL, dilution 1:180.
5. Antibody diluent buffer: 12ml.
6. Streptavidin-HRP: 300µL, dilution 1:40.
7. Streptavidin-HRP diluent buffer: 12ml.
8a &8b Chromogen-A &B: 6ml each.
9. Stop Solution: 6ml.