Data sheet
| Background | Interleukin-1β ( IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion. |
| Alternate Names | IL1B, IL-1, IL1-BETA, IL1F2, interleukin 1 beta |
| Gene ID | 16176 |
| UniProt ID | P10749 |
More info
Product name
Mouse IL-1beta ELISA Kit
Catalog No.
FM-E100055
Size
96T
Range
31.2pg/mL-2000pg/mL
Sensitivity
25 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
serum, plasma, body fluids or cell culture supernates.
Kit Components
1. Lyophilized recombinant mouse IL-1βstandard: 2ng/tube-2.
2. One 96-well plate precoated with anti- mouse IL-1β antibody.
3. Sample diluent buffer: 25 ml
4. Biotinylated anti- mouse IL-1β antibody : 60µL, dilution 1:180.
5. Antibody diluent buffer: 12ml.
6. Streptavidin-HRP: 300µL, dilution 1:40.
7. Streptavidin-HRP diluent buffer: 12ml.
8a &8b Chromogen-A &B: 6ml each.
9. Stop Solution: 6ml.