More info
Assay Range | 0-600 ng/ml |
Sensitivity | 1.0 ng/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse Ceruloplasmin standard: 2 vials
2. One 96-well plate coated Mouse Ceruloplasmin Ab
3. Diluent buffer (5x): 12 ml
4.HRP conjugated Ab: 1 vial
5. TMB developing reagent : 12 ml.
6. Stop solution: 12 mL x1
7. Washing solution (20x): 25 mL x1
Background
Ceruloplasmin , also known as caeruloplasmin, is a multifunctional protein encoded by the CP gene in humans. Ceruloplasmin is the major copper-carrying protein in the blood, itcarries more than 95% of the total copper in healthy human plasma. It provides Cu2+ ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. In addition, it has ferroxidase activity oxidizing Fe2+ to Fe3+ without releasing radical oxygen species. It is involved in iron transport across the cell membrane.