More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse MMP-12 standard: 2 vials
2. One 96-well plate precoated with anti- Mouse MMP-12 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases which can degrade the extracellular matrix. Matrix metalloproteinase-12 (MMP-12), also known as macrophage elastase (ME), is a member of the MMP family and is encoded by the MMP-12 gene in humans. Pro-MMP-12 is composed of a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. Expression of MMP-12 in monocytes can be induced by cytokines such as GM-CSF and CD40 signaling. In addition to elastin, MMP-12 has a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, α1-antitrypsin, and plasminogen. MMP-12 is required for macrophage-mediated proteolysis and matrix invasion in mice. MMP-12 may play a role in the pathogenesis of aortic aneurysms and in the development of pulmonary emphysema that results from chronic inhalation of cigarette smoke. In addition, MMP-12 can also activate MMP-2 and MMP-3.