| Assay principle |
The Human Phospho-Axl ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti- Human Phospho-Axl specific antibody has been pre- coated onto 96-well plate. Human Phospho-Axl present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Human Phospho-Axl detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Human Phospho-Axl captured on plate.
Materials supplied |
