Data sheet
| Assay Range | 62.5 - 4000 pg/ml |
More info
Sensitivity
60 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Flu B HA standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-Flu B HA Ab
3. Sample diluent buffer: 12 mL x 2
4. HRP conjugated anti-Flu B HA Ab: 1 vial, dilution 1:60.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Influenzavirus B is a genus in the virus family Orthomyxoviridae. Influenza B viruses are only known to infect humans and seals, giving them influenza. Influenza B viruses cause the same spectrum of disease as influenza A. However, influenza B viruses do not cause pandemics. Influenza B mutates at a rate 2-3 times slower than type A and consequently is less genetically diverse, with only one influenza B serotype.
