Data sheet
| Assay Range | 46.88 - 3000 pg/ml |
More info
Sensitivity
40 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant H7N7 HA standard: 3 ng/vial - 2.
2. One 96-well plate precoated with anti-H7N7 HA Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-H7N7 HA Ab: 1 vial, dilution 1:250.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
H7N7 is a subtype of Influenzavirus A, a genus of Orthomyxovirus, the viruses responsible for influenza. H7N7 can infect humans, birds, pigs, seals, and horses in the wild; and has infectedmice in laboratory studies. H7N7 represents a pandemic threat because influenza H7N7 viruses do not commonly infect human, there is little or no antibody protection against these viruses in the human population. Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. HA plays a major role in the determination of host range restriction and virulence.
