Data sheet
| Assay Range | 156 - 10000 pg/ml |
| Sensitivity | 100 pg/ml |
| Storage | Store at 2 - 8⁰C. Keep Standard and detection antibody at -20⁰C. |
| Expiration | Six months at 2 - 8 ⁰C. |
| Application | For quantitative determination of Influenza A H9N2 Hemagglutinin. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Sample type | Serum, plasma, body fluids. |
| Solubility | 96T |
More info
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant H9N2 hemagglutinin standard: 10 ng/vial-2.
2. One 96-well plate precoated with anti-H9N2 hemagglutinin antibody.
3. Sample diluent buffer: 12 ml
4. HRP conjugated anti-H9N2 hemagglutinin antibody: 1 vial, dilution 1:2500.
5. Detection Antibody diluention buffer: 12ml.
6. Assay diluent buffer: 12 ml.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Influenza virus A is a genus of the Orthomyxoviridae family of viruses. Influenza A virus causes influenza in birds and some mammals and is the only species of influenza virus A. Strains of all subtypes of influenza A virus have been isolated from wild birds, although disease is uncommon. Some isolates of influenza A virus cause severe disease both in domestic poultry and, rarely, in humans. Occasionally, viruses are transmitted from wild aquatic birds to domestic poultry, and this may cause an outbreak or give rise to human influenza pandemics.
