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H5N1 ( Avian Flu ) Hemagglutinin ELISA Kit

NS-E10004

$576.00

Data sheet

Assay Range 125 - 8000 pg/ml
Sensitivity 50 pg/ml
Size 96T
Storage Store at 2 - 8⁰C. Keep Standard and detection antibody at -20⁰C.
Expiration Six months at 2 - 8 ⁰C.
Application For quantitative determination of hemagglutinin.
Assay principle This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA).
Sample type Serum, plasma, body fluids.

More info

 

Sample Volume
100 µL final volume, dilution factor varies on samples
 
Detection Method
Chromogenic 
 
 
Kit Components
 
 
 
1. Recombinant H5N1 hemagglutinin standard:8 ng/vial-2.
 
 
2. One 96-well plate precoated with anti-H5N1 hemagglutinin antibody.
 
 
3. Sample diluent buffer: 12 ml x 2
 
 
4. HRP- conjugated anti-H5N1 hemagglutinin antibody:1 vial, dilution 1:250.
 
 
5. Detection Antibody diluention buffer: 12ml 
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.
 
  
 

 

Background

 

Influenza A virus subtype H5N1, also known as A(H5N1) or simply H5N1, is a subtype of the influenza A virus which can cause illness in humans and many other animal species. H5N1 is a highly pathogenic avian (bird) flu virus that has caused serious outbreaks in domestic poultry in parts of Asia and the Middle East. Highly pathogenic refers to the virus’s ability to produce disease.. Like all other influenza A subtypes, the H5N1 subtype is an RNA virus. It has a segmented genome of eight negative sense, single-strands of RNA, abbreviated as PB2, PB1, PA, HA, NP, NA, MP and NS. HA codes for hemagglutinin, an antigenic glycoprotein found on the surface of the influenza viruses and is responsible for binding the virus to the cell that is being infected. Hemagglutinin plays a major role in the determination of host range restriction and virulence because human influenza HA preferentially binds to SA-α-2,6 while avian influenza HA preferentially binds to SA-α-2,3.

 


 
 
Product Citation:
 
1. Direct immunosensing of avian influenza A virus in whole blood using hybrid nanocomposites
John Buozis, Syed Rahin Ahmed, Rohit Chand, Éva Nagy, Suresh Neethirajan
bioRxiv 343376; doi: https://doi.org/10.1101/343376
 

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