> >>Cynomolgus p53 / TP53 ELISA Kit
View larger

Cynomolgus p53 / TP53 ELISA Kit

NS-E10262

$576.00

Data sheet

Assay Range 140.6 - 9000 pg/ml

More info

Sensitivity
140 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant Cynomolgus p53 standard: 9 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-Cynomolgus p53 Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Cynomolgus p53 Ab: 1 vial, dilution 1:200.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

Tumor protein p53, also known as p53, cellular tumor antigen p53 or transformation-related protein 53 (TRP53), is classified as a tumor suppressor gene. p53 is crucial in multicellular organisms, where it prevents cancer formation, thus, functions as a tumor suppressor. p53 plays a role in conserving stability by preventing genome mutation. TP53 gene is the most frequently mutated gene (>50%) in cancer, indicating that the TP53 gene plays a crucial role in preventing cancer formation. Activated p53 binds DNA and activates expression of several genes including microRNA miR-34a, WAF1/CIP1 encoding for p21 and hundreds of other down-stream genes. p21 (WAF1) binds to the G1-S/CDK (CDK4/CDK6, CDK2, and CDK1) complexes (molecules important for the G1/S transition in the cell cycle) inhibiting their activity.

Standard Curve

© 2024 Novateinbio.com