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This matched Ab set contains the capture and detection antibodies required for the development of sandwich immunoassays to detect/ quantify TNF-α (TNF-alpha) in serum/ cell culture supernatants.
Optional: Biotinylated detection antibody
Applications: ELISA, IP-WB, FRET/TR-FRET, AlphaLISA/AlphaScreen, ultrasensitive PLA(proximity ligation assay), cell- based RCA (rolling circle amplification), Luminex and other bead-based assays
Reactivity: Human
Assay Range: 15.6 pg/mL to 1000 pg/mL (as determined by sandwich ELISA)
Kit Contents: Reagents are sufficient for atleast 5 X 96-well plates
Capture Antibody: Mouse anti-human TNF-α (50 μg)
Detection Antibody: Rabbit anti-human TNF-α (5 μg)