Interleukin-2 Human Recombinant ( IL 2 Human )

Data sheet

Formulation

The protein (1mg/ml) was lyophilized after extensive dialysis against 20mM PBS pH=7.4.

Solubility

It is recommended to reconstitute the lyophilized Interleukin 2 in 20mM AcOH not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Interleukin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15kDa. The IL-2 is purified by proprietary chromatographic techniques.

Protein Background

IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of

Expression host

Escherichia Coli.

Synonyms

T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to

Amino acid sequence

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is

References

1.Title:CD8 Kinetically Promotes Ligand Binding to the T-Cell Antigen Receptor.Publication:Biophysical Journal Volume 89 September 2005 21212133.Link:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1366714/pdf/2121.pdf2.Title: Detection of expressed IL-32 in