4-1BB Receptor Human Recombinant ( 4 1BBR Human )

Data sheet

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

Solubility

It is recommended to reconstitute the lyophilized 4-1BB Receptor in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Description

4-1BB Soluble Receptor Recombinant Human also called Tumor necrosis factor receptor superfamily member 9 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids, having a molecular mass of 17718 Dalton and containing

Protein Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induc

Expression host

Escherichia Coli.

Synonyms

Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor T-cell, antigen 4-1BB homolog, T-cell antigen ILA, CD137 antigen, CDw137, ILA, 4-1BB, MGC2172, 4-1BBR, TNFRSF9.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized 4-1BB Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution 4-1BBR should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommende

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Arg-Thr-Arg.

Biological Activity

The activity was determined by the inhibition of 4-1BB ligand mediated stimulation of IL-8 production by human PBMC. Results: 90% inhibition using 1ug for both 4-1BB ligand and 4-1BB receptor.