B lymphoid tyrosine kinase Human Recombinant ( BLK Human )

Data sheet

Formulation

BLK protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1M NaCl, 1mM DTT and 0.1mM PMSF.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Description

BLK Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 525 amino acids (1-505 a.a.) and having a molecular mass of 59.8kDa.BLK is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromat

Protein Background

B lymphoid kinase (BLK) is a 55kDa tyrosine kinase with SH3, SH2, and catalytic domains which contain consensus sequences of the Src protein tyrosine kinase family. BLK is a nonreceptor tyrosine-kinase belonging to the src family of proto-oncogenes which

Expression host

Escherichia Coli.

Synonyms

Tyrosine-protein kinase Blk, B lymphocyte kinase, p55-Blk, BLK, MODY11, MGC10442.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI RESETNKGAFSLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA LVQHYSKKGD GLC

Please note

ELISA. Inhibition Assays. Western Blotting.