Mucosae-Associated Epithelial Chemokine Human Recombinant ( CCL28 ) ( CCL28 Human )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM PBS and 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized CCL28 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

CCL28 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 108 amino acids and having a molecular mass of 12.3 kDa. The CCL28 is purified by proprietary chromatographic techniques.

Protein Background

CCL28 is part of the subfamily of small cytokine CC genes. CCL28 shows chemotactic activity for resting CD4 or CD8 T cells and eosinophils. CCL28 binds to chemokine receptors CCR3 and CCR10. CCL28 is involved in the physiology of extracutaneous epithelial

Expression host

Escherichia Coli.

Synonyms

MEC, CCK1, SCYA28, MGC71902, CCL28, C-C motif chemokine 28, Small-inducible cytokine A28, Mucosae-associated epithelial chemokine, Protein CCK1.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized CCL28 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL28 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a

Amino acid sequence

SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY.

Biological Activity

Determined by its ability to chemoattract human lymphocytes using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

Antibody Properties

Reconstitute with of H2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Available Conjugates

Blocking, staining and activating antibody. For staining, use 10ul/1,000,000 cells. Titer for blocking and activating T cells should be determined by the investigator.

Immunological function

This antibody is also available conjugated to biotin and FITC.