CD14 Human Recombinant, CHO ( CD14 Human, CHO )

Data sheet

Formulation

CD14 was lyophilized from a concentrated protein solution (1.0 mg/ml) containing phosphate-buffered saline, pH 7.2.

Solubility

It is recommended to reconstitute the lyophilized CD14 in sterile 18M-cm H2O not less than 100ug/ml. Further dilutions should be made with phosphate buffered saline (PBS).

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

The CD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The myeloid differentiation antigen CD14 acts as the major receptor for bacteri

Protein Background

CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity rec

Expression host

CHO-cells.

Synonyms

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD14 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a ca

Biological Activity

Up to 20 ug/ml CD14 inhibit binding of FITC-LPS (0.5ug/ml) to 600,000 CD14+CHO transfectants (FACS).